Useful Links & Tools
- MSDS
- Certificate of Analysis
- A7550 - Enzyme Assay (22 KB)
- Bulk Quote
- Similar Products
A7550
Sigma
Achromopeptidase from Achromobacter lyticus
lyophilized powder, Protein ~5 % by biuret, 300-600 units/mg solid
| CAS Number: | 123175-82-6 |
| Enzyme Commission (EC) Number: | 3.4.21.50 ( BRENDA | IUBMB ) |
| MDL number: | MFCD00130333 |
Description
| Unit Definition | One unit will produce a change in A600 of 0.001 per minute per mL at pH 8.0 at 37 °C using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path). |
| Physical form | Crude powder containing salts and medium components |
| Biochem/physiol Actions | Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. pH Optimum for activity: pH 8.5 - 9 Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD600=0.6 over 2 hours at 37 °C. |
Properties
| form | lyophilized powder |
| composition | Protein, ~5% biuret |
| foreign activity | Collagenase, present |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 36 |
| WGK Germany | 3 |
References
| reference | Ezaki, T and Suzuki, S. J. Clin. Microbiol. 16, 844-846 , (1982) |
